Medical microbiology Assignment Example | Topics and Well Written Essays - 750 words

Medical microbiology - Assignment ExampleSome of them are insufficient media, bactericidal activity present in strain, amount of channel used for culturing, antibiotics occurrence, blood collection time. Most of bacterial cells of Salmonella serovar Typhi resides intracellulary in the patients blood.There is a need of reliable, rapid and tenuous methods for clinical detection of Salmonella serovar Typhi. Serological tests are available and its sensitivity and specificity is very low. The blood ending sensitivity is highest during the first week of illness but it then gradually decreases with advancement of illness. 45- 70% patients of typhoid fever can be identified through blood culturing. It depends upon the blood sample amount, bacteraemic level of Salmonella, assimilation medium type, length of incubation.Also the Salmonella bacteria presence in patients blood is very low, often below detection by blood culturing and even below detection by PCR. Difficulties become more due to limited amount of patient blood sample.Ox gall tryptone soy caudex was used for blood culture it allows blood cell lysis completely, which causes the release of intracellular bacteria. It does not inhibit the exploitation of Salmonella Typhi. From research it was found out that bacterial growth can be increased if blood culture media contains lyzing agents for blood cells. By performing various tests it was found out that 2.4% ox bile presence in blood culture will lyse blood cells within 1.5 hours so that the bacteria present intracellulary came out.Salmonella bacteria when kept in tryptone soya broth containing 2.4% ox bile for 3 hours will increase the amount of bacteria from 0.75 CFU per milliliter of blood. This level can be easily detectible by using regular PCR method. This blood culture PCR assay processing time is lesser than 8 hours as compared to conventional blood culture which may take 2 -5 days.Salmonella serovars patients blood or serum is also bactericidal